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Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. Molecular Biomethods Handbook, 2nd Edition expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Part A of the book describes nucleic acid methods, such as gene expression profiling, microarray analysis and quantitative PCR. In Part B, protein and cell-based methods are outlined, in subjects ranging from protein engineering to high throughput screening. Written by a well-established panel of research scientists, Molecular Biomethods Handbook, 2nd Edition provides an up to date collection of methods used regularly in the authors ̕own research programs. Comprehensive collection of wide-ranging molecular and biochemical techniques frequently used in medical and clinical research laboratories around the world Covers a wide array of techniques and protocols used for diagnostics Includes step-by-step reproducible laboratory protocols written by experts in the field and includes a notes section that outlines tricks of the trade

Índice: From the contents Part A: Nucleic Acid Biomethods: The manipulation of nucleic acids: basic tools and techniques.- Restriction Enzymes.- Principles and Medical Applications of the Polymerase Chain Reaction.- Probe Design, Production, and Applications.- Southern Blotting as a Diagnostic Method.- Introduction to Capillary Electrophoresis of DNA.- Denaturing High-Performance Liquid Chromatography (DHPLC) for nucleic acid analysis.- Denaturing Gradient Gel Electrophoresis (DGGE).- Single Strand Conformation Polymorphism (SSCP) Analysis.- Randomly amplified polymorphic DNA (RAPD); a useful tool for genomic char... Etc.