Practical biotransformations : a beginner's guide / Gideon GroganMaterial type: Book; Format: print Series: Postgraduate chemistry.Publisher: West Sussex : Wiley-Blackwell, 2009Description: XII, 331 p. : il. ; 24 cm.ISBN: 978-1-4051-9367-2.Subject(s): Química orgánica | Biotecnología | Biosíntesis | Enzimas
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This book has been written with two primary aims; to demystify this research area in an accessible and user-friendly way, and to encourage the introduction of biocatalysis into synthetic organic labs. The book is aimed at advanced undergraduate and postgraduate organic chemists and researchers in both academia and industry to make them aware of potential value of biotransformation methodology, and who do not know how to begin to implement the techniques in the laboratory. There is a clear emphasis on usability, helping the reader to assemble the necessary equipment and resources to begin research in the biotransformations area. The book is intended as a user-friendly manual which can be referred to on a daily basis in the wet lab. Topics covered include: Introduction to equipment in a biotransformations lab easily incorporated into an established organic chemistry lab. Biocatalyst sources descriptions of currently available enzymes and microbiological culture collections with contact information. Advantages of using powdered commercially available enzymes with examples from the literature. The use of microbial biocatalysts, including maintenance and growth. Basic gene cloning and the use of designer biocatalysts. Industrial case study as an illustration of a bench-level to process-level reaction. This book is part of the Postgraduate Chemistry series.