Environmental genomics / edited by C. Cristofre Martin

Colaborador(es): Martin, C. Cristofre [editor]Tipo de material: TextoTextoSeries Methods in molecular biology ; 410Detalles de publicación: Totowa : Humana Press, 2008 Descripción: XI, 364 p. : il. ; 24 cmISBN: 9781588297778Tema(s): GenómicaResumen: The goal of Environmental Genomics is to serve as a manual for an environmental scientist who wishes to embrace genomics to answer environmental questions. The volume is divided into the sections: gene expression profiling, whole genome and chromosome mutation detection, and methods to assay genome diversity and polymorphisms within a particular environment. The studies presented will reduce the uncertainties associated with environmental risk assessment and provide a systematic framework for determining environmental impact and ensuring human health and the sustainability of natural populations. Concise protocols to conduct genomic and molecular biology analysis Trouble shooting guideResumen: Índice: From the contents High-throughput Whole Mount in situ Hybridization of Zebrafish Embryos for Analysis Tissue Specific Gene Expression Changes Following Environmental Perturbation.- Fluorescent RNA Arbitrarily Primed (FRAP) PCR - A New Differential Display Approach to Detect Contaminant-Induced Alterations of Gene Expression In Wildlife Species.- Isolation of O3-response Genes from Arabidopsis thaliana using cDNA Macroarray.- Use of cDNA Macroarrays and Gene Profiling For Detection of Effects by Environmental Toxicants.- Constructing and Screening a cDNA library: Methods for Identification and Characterizat... Etc.
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The goal of Environmental Genomics is to serve as a manual for an environmental scientist who wishes to embrace genomics to answer environmental questions. The volume is divided into the sections: gene expression profiling, whole genome and chromosome mutation detection, and methods to assay genome diversity and polymorphisms within a particular environment. The studies presented will reduce the uncertainties associated with environmental risk assessment and provide a systematic framework for determining environmental impact and ensuring human health and the sustainability of natural populations. Concise protocols to conduct genomic and molecular biology analysis Trouble shooting guide

Índice: From the contents High-throughput Whole Mount in situ Hybridization of Zebrafish Embryos for Analysis Tissue Specific Gene Expression Changes Following Environmental Perturbation.- Fluorescent RNA Arbitrarily Primed (FRAP) PCR - A New Differential Display Approach to Detect Contaminant-Induced Alterations of Gene Expression In Wildlife Species.- Isolation of O3-response Genes from Arabidopsis thaliana using cDNA Macroarray.- Use of cDNA Macroarrays and Gene Profiling For Detection of Effects by Environmental Toxicants.- Constructing and Screening a cDNA library: Methods for Identification and Characterizat... Etc.

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