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Practical methods for biocatalysis and biotransformations / editors John Whittall, Peter W.Sutton

Contributor(s): Whittall, John [] | Sutton, Peter W [].
Material type: materialTypeLabelBook; Format: print Publisher: West Sussex : Wiley, 2009Description: XXIX, 402 p. ; 25 cm.ISBN: 978-0-470-51927-1.Subject(s): Enzimas -- Biotecnología | Biotransformación (Metabolismo) | Síntesis orgánica | Biosíntesis
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Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a huge choice of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities. Practical Methods for Biocatalysis and Biotransformations is a how-to guide focussing on commercially available enzymes and strains of microorganisms that are readily obtained from culture collections. The source of starting materials and reagents, hints, tips and safety advice (where appropriate) are given to ensure, as far as possible, that the procedures are reproducible. Comparisons to alternative methodology are given and relevant references to the primary literature are cited. Contents include: perspectives of biotransformations in small molecule pharmaceutical development biocatalyst identification and scale-up: molecular biology for chemists kinetic resolutions using biotransformations dynamic kinetic resolution for the synthesis of esters, amides and acids using lipases enzymatic selectivity in synthetic methods aldolase enzymes for complex synthesis enzymatic synthesis of glycosides and glucuronides. synthesis of cyanohydrins using hydroxynitrile lyases synthesis of chiral sec-alcohols by ketone reduction reduction of functional groups enzymatic oxidation chemistry whole cell oxidations and dehalogenations Practical Methods for Biocatalysis and Biotransformations is an essential text offering validated biocatalyst methods for researchers and postgraduate students in industry and academia.

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