Cell culture techniques / edited by Michael Aschner, Cristina Suñol, Anna Bal-Price
Tipo de material:
TextoSeries Neuromethods ; 56Detalles de publicación: London : Humana Press, 2011 Descripción: XIV, 497 p. : il. ; 26 cmISBN: 978-1-61779-076-8Tema(s): Células -- Cultivo | Citología técnica | Neurotoxicología | Neurociencias -- MetodologíaResumen: Societal, ethical, and cost-related issues, not to mention the need for sound scientific methods, have led to new and refined methods for the evaluation of health risks associated with neurotoxic compounds, relevant and predictive of exposure, relatively inexpensive, and ideally amenable to high throughput analysis and a reduction in animal use. Cell Culture Techniques presents thorough traditional chapters, such as those on various cell culture methods that have evolved over the years, as well as innovative approaches to neurotoxicologic testing. Accordingly, this detailed volume describes how stem cells, computational biology, and other novel powerful methods can now be applied to address the challenges of neurotoxic testing. As part of the Neuromethods series, this work provides the kind of intensive description and implementation advice that is crucial for getting optimal results in the laboratory. Practical and authoritative, Cell Culture Techniques serves both the novice and the experienced neurotoxicologist by inspiring the further development of mechanistically-driven, cost-effective, high throughput series of tests needed to meet the many contemporary challenges. Facilitates the study of adverse changes in the developing and mature brain alike. Features classic as well as innovative new approaches to neurotoxicologic testing. Aids in the reduction of the necessity of animal testing.Resumen: Índice: Guidance on Good Cell Culture Practice (GCCP). Induced PluripotentStem Cells (iPSCs): An Emerging Model System for the Study of Human Neurotoxicology. Neural Stem Cells. Primary Cultures for Neurotoxicity Testing. Preparation and Use of Serum-Free Aggregating Brain Cell Cultures for Routine Neurotoxicity Screening. Cell Culture to Investigate Neurotoxicity and Neurodegeneration Utilizing Caenorhabditis elegans. Modeling the Blood-Brain Barrier. In vitro Models of the Blood-Cerebrospinal Fluid Barrier and Their Use in Neurotoxicological Research. Introducing Cloned Genes into Cultured Neurons Providing Nov... Etc.
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Societal, ethical, and cost-related issues, not to mention the need for sound scientific methods, have led to new and refined methods for the evaluation of health risks associated with neurotoxic compounds, relevant and predictive of exposure, relatively inexpensive, and ideally amenable to high throughput analysis and a reduction in animal use. Cell Culture Techniques presents thorough traditional chapters, such as those on various cell culture methods that have evolved over the years, as well as innovative approaches to neurotoxicologic testing. Accordingly, this detailed volume describes how stem cells, computational biology, and other novel powerful methods can now be applied to address the challenges of neurotoxic testing. As part of the Neuromethods series, this work provides the kind of intensive description and implementation advice that is crucial for getting optimal results in the laboratory. Practical and authoritative, Cell Culture Techniques serves both the novice and the experienced neurotoxicologist by inspiring the further development of mechanistically-driven, cost-effective, high throughput series of tests needed to meet the many contemporary challenges. Facilitates the study of adverse changes in the developing and mature brain alike. Features classic as well as innovative new approaches to neurotoxicologic testing. Aids in the reduction of the necessity of animal testing.
Índice: Guidance on Good Cell Culture Practice (GCCP). Induced PluripotentStem Cells (iPSCs): An Emerging Model System for the Study of Human Neurotoxicology. Neural Stem Cells. Primary Cultures for Neurotoxicity Testing. Preparation and Use of Serum-Free Aggregating Brain Cell Cultures for Routine Neurotoxicity Screening. Cell Culture to Investigate Neurotoxicity and Neurodegeneration Utilizing Caenorhabditis elegans. Modeling the Blood-Brain Barrier. In vitro Models of the Blood-Cerebrospinal Fluid Barrier and Their Use in Neurotoxicological Research. Introducing Cloned Genes into Cultured Neurons Providing Nov... Etc.
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