TY  - BOOK
AU  - Amberg,David C.
AU  - Burke,Dan
AU  - Strathern,Jeffrey N.
AU  - Burke,Dan
ED  - Cold Spring Harbor Laboratory.
TI  - Methods in yeast genetics: a Cold Spring Harbor Laboratory course manual 
SN  - 0879697288 (pbk. : alk. paper)
PY  - 2005///
CY  - Cold Spring Harbor, N.Y.
PB  - Cold Spring Harbor Laboratory Press
KW  - Yeast fungi
KW  - Genetics
KW  - Laboratory manuals
KW  - Yeasts
KW  - genetics
KW  - Laboratory Manuals
KW  - Genética molecular
KW  - Manuales de laboratorio
KW  - Levaduras
N1  - Rev. ed. of: Methods in yeast genetics / Dan Burke, Dean Dawson, Tim Stearns, 2000 ed. 2000; Includes bibliographical references and index; EXPERIMENTS    I. Looking at Yeast Cells    II. Isolation and Characterization of Auxotrophic, Temperature-sensitive, and Osmotic-sensitive Mutants    III. Meiotic Mapping    IV. Mitotic Recombination and Random Spore Analysis    V. Transformation of Yeast    VI. Synthetic Lethal Mutants    VII. Gene Replacement    VIII. Isolation of ras2 Suppressors    IX. Manipulating Cell Types    X. Isolating Mutants by Insertional Mutagenesis    XI. Isolation of Separation of Function Mutants by Two-hybrid Differential Interaction Screening     TECHNIQUES AND PROTOCOLS    1. High-efficiency Transformation of Yeast    2.  Quick and Dirty  Plasmid Transformation of Yeast Colonies    3. Yeast DNA Isolations    A. Yeast DNA Miniprep (40 ml)    B. Yeast DNA Miniprep (5 ml)    C. A Ten-minute DNA Preparation from Yeast    D. Yeast Genomic DNA: Glass-bead Preparation    4. Yeast Protein Extracts    5. TAP Purification    6. Yeast RNA Isolations    7. Hydroxylamine Mutagenesis of Plasmid DNA    8. Assay of  b2;-Galactosidase in Yeast    9. Plate Assay for Carboxypeptidase  a5;    10. Random Spore Analysis    11. Yeast Vital Stains    A. DAPI Stain of Nuclear and Mitochondrial DNA    B. Visualizing Mitochondria with DiOC6 or DilC5(3)    C. Visualizing Vacuoles and Endocytic Compartments with FM4-64    D. Calcofluor Staining of Chitin and Bud Scars    12. Yeast Immunofluorescence    13. Actin Staining in Fixed Cells    14. PCR-mediated Gene Disruption    A. One-step PCR Gene Disruption    B. Gene Disruption by Double-fusion PCR    15. Yeast Colony PCR    16. Measuring Yeast Cell Density by Spectrophotometry    17. Cell Synchrony    18. Chromatin Immunoprecipitation    19. Flow Cytometry of Yeast DNA    20. Logarithmic Growth    21. EMS Mutagenesis    22. Tetrad Dissection    23. Making a Tetrad Dissection Needle    24. Picking Zygotes    25. Determining Plating Efficiency    26. DNA Miniprep from E. coli    27. Preparing and Transforming Competent E. coli    28. Storing and Handling of the Systematic Deletion Collection     APPENDICES
ER  -