TY  - BOOK
AU  - Westermeier,Reiner
AU  - Naven,Tom
AU  - Hopker,Hans-Rudolf
TI  - Proteomics in practice: a guide to successful experimental design 
SN  - 9783527319411
PY  - 2008///
CY  - Weinheim
PB  - Wiley-VCH
KW  - Proteómica
KW  - Proteinas
N2  - till the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR.Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website
ER  -