Instrumental Development Attachable to High Magnification Microscopes for Obtaining Totally Focalized Images [Recurso electrónico] / Francisco Javier Navas Pineda, M. Kulawik, Joaquín Martín Calleja
Tipo de material:
TextoSeries WOS | Artículos científicos UCADetalles de publicación: 2008 Tema(s): Optical Focusing | Optical Images | Optical Microscopes | Optical Microscopy | Laser-Scanning Microscopy | Confocal Microscopy | Optical Microscopy | Depth | Focus | Microstructure | Apoptosis
En: Review of Scientific InstrumentsResumen: A complete system (instrumentation and software) for acquiring micrograph images with an extended depth of focus and with no alteration to the original chromaticity has been developed. The buildup system includes (a) the electromechanical components to be coupled to a commercial microscope in order to improve it, (b) a computer program with which the focusing distance can be micrometrically modified at the same time that a set of digital images can be acquired, and (c) software that permits the in-focus pixels of each image to be extracted and incorporated into a global image with an extended depth of focus greater than that supplied by the optical characteristics of the objective used to visualize the sample. The algorithm used does not have any influence on the sample chromaticity and the generated image can be obtained with full chromaticity.
A complete system (instrumentation and software) for acquiring micrograph images with an extended depth of focus and with no alteration to the original chromaticity has been developed. The buildup system includes (a) the electromechanical components to be coupled to a commercial microscope in order to improve it, (b) a computer program with which the focusing distance can be micrometrically modified at the same time that a set of digital images can be acquired, and (c) software that permits the in-focus pixels of each image to be extracted and incorporated into a global image with an extended depth of focus greater than that supplied by the optical characteristics of the objective used to visualize the sample. The algorithm used does not have any influence on the sample chromaticity and the generated image can be obtained with full chromaticity.
En:Review of Scientific Instruments (2008, vol. 79, n. 11
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